Caenorhabditis elegans deep lipidome profiling by using integrative mass spectrometry acquisitions reveals significantly altered lipid networks / 药物分析学报

Lipidomics coverage improvement is essential for functional lipid and pathway construction.A powerful approach to discovering organism lipidome is to combine various data acquisitions,such as full scan mass spectrometry(full MS),data-dependent acquisition(DDA),and data-independent acquisition(DIA).Caenorhabditis elegans(C.elegans)is a useful model for discovering toxic-induced metabolism,high-throughput drug screening,and a variety of human disease pathways.To determine the lipidome of C.elegans and investigate lipid disruption from the molecular level to the system biology level,we used integrative data acquisition.The methyl-tert-butyl ether method was used to extract L4 stage C.elegans after exposure to triclosan(TCS),perfluorooctanoic acid,and nanopolystyrene(nPS).Full MS,DDA,and DIA integrations were performed to comprehensively profile the C.elegans lipidome by Q-Exactive Plus MS.All annotated lipids were then analyzed using lipid ontology and pathway analysis.We annotated up to 940 lipids from 20 lipid classes involved in various functions and pathways.The biological in-vestigations revealed that when C.elegans were exposed to nPS,lipid droplets were disrupted,whereas plasma membrane-functionalized lipids were likely to be changed in the TCS treatment group.The nPS treatment caused a significant disruption in lipid storage.Triacylglycerol,glycerophospholipid,and ether class lipids were those primarily hindered by toxicants.Finally,toxicant exposure frequently involved numerous lipid-related pathways,including the phosphoinositide 3-kinase/protein kinase B pathway.In conclusion,an integrative data acquisition strategy was used to characterize the C elegans lipidome,providing valuable biological insights into hypothesis generation and validation.

The immuno-depressive situation in patients with cancer and preliminary results of treatment protocol with aslem as adjuvant after surgery

From March 2000 to October 2001, 26 cancer patients of the cardiac region were registered to our study. Most patients were in advanced stage, apparently showed an impaired immunity both local and systemic. 20/21 (95%) cases of positive mutant P53 protein were detected at the tumor. Post operative adjuvant therapy with Aslem tend to increase the survival rate after resection (60.61% of the treated group compared with 33.33% of the untreated group)

Review of head trauma management in People hospital 115 in Ho Chi Minh during 1992- 1996

Basing on statistics of head trauma during the period 1991-1996, the authors stress on the increasing number of patients from year to year. They put on remarks about the organisation of head trauma emergency, which is an acute problem to be solved.